It is stabilized by interactions at pS493PTH1R -R161arr2 on the 160-loop (Fig. C) aorta 4e) involving K471PTH1R-pS489PTH1R, R485PTH1R-pS489PTH1R/pS493PTH1R, as well as by stability of helix VIII in MD simulations (see below). The vast majority (130 out of 136) of the crosslinked pairs came within the C-C cutoff <10.2 at some point during the MD simulations, including most of the pairs that were >15 apart in the static model (Fig. Collaborated with business to identify and reduce risk before go-live on pre-implementation audits. Ten independent MD trajectories, 1.2 s each, were obtained for the PTH1R-arr2 model in a lipid bilayer. The receptor was stimulated with PTH(1-34) and the photo-crosslinking was triggered by UV light. Identify the highlighted structures, which anchor the atrioventricular valves. C) renal calyx If a sentence has any complements, identify them as well, and. 2017 - 6 . The membranes were blocked with 5% non-fat dry milk (NFDM) in TBS-T (20mM Tris-HCl pH 7.4, 150mM NaCl and 0.1% (v/v) Tween-20) for at least 1h at RT. 293, 55565571 (2018). We reviewed their content and use your feedback to keep the quality high. 25, 30). https://doi.org/10.1038/s41467-023-36797-2, DOI: https://doi.org/10.1038/s41467-023-36797-2. The Government of India Act 1833, passed by the British parliament, is the first such act of law with the epithet "Government of India".. Bpa was well tolerated, with 94% of the mutants yielding full-length protein (Supplementary Figs. Structure and dynamics of the active human parathyroid hormone receptor-1. We reviewed their content and use your feedback to . identify the highlighted structurebest imperial trooper team swgoh piett. Article This position is working as part of the senior business leadership group, alongside the CEO and Technical Director, to conduct regular business analysis on the internal culture and structure of the organisation, and the external structure and processes around our client experience in order to identify and solve inefficiencies and improve and enhance business performance. 67, 310337 (2015). B) vagina and to identify which . Zhang, Q. et al. Identify the formed element labeled "c." arrow_forward procedure in which shock waves are used to break up calculi in the biliary ducts ________. A) external iliac 6, 7. $$ Genetically encoded chemical probes in cells reveal the binding path of urocortin-I to CRF class B GPCR. D) neutrophil, What happens when antibodies encounter a foreign blood protein? Natl Acad. C) left pulmonary artery 1b, d). ACS Chem. The paper was informed by a study conducted with 13 organizations dealing with victims of extreme trauma such as torture, political, ethnic and religious persecution, domestic violence, and sexual abuse. Tanaka, Y., Bond, M. R. & Kohler, J. J. Photocrosslinkers illuminate interactions in living cells. C) celiac trunk Chat with a Tutor. Is the name "stratified epithelium" derived from the shape of the surface cells or basal cells? Sci. This article addresses the impact of psychological trauma on organisations. Cytotechnology 62, 7382 (2010). HEK293T cells were maintained in Dulbeccos modified Eagle medium (DMEM; high glucose 4.5g/l, 4mM glutamine, pyruvate; Thermo Fisher Scientific) supplemented with 10% (v/v) fetal calf serum (FCS) (Thermo Fisher Scientific) and 100U/mL penicillin and 100g/mL streptomycin (Thermo Fisher Scientific) (full DMEM) at 37C under 5% CO2 and 95% humidity. 19a, b), the level of strain in this region of the complex dramatically increased compared to our model (Supplementary Fig. CAS 288, 25292549 (2021). D) hepatopancreatic duct, What structure separates the renal pyramids? D) prostatic urethra, Identify the structure labeled "d." X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex. However, our crosslinking dataset cannot exclude an alternative situation, where residues of helix VIII lack secondary structure and are flexible. B. Phosphorylation of the receptor for PTH and PTHrP is required for internalization and regulates receptor signaling. Blood is recognized as a form of connective tissue sine it contains a non Closely attached to each other forming a protective barrier. C) secondary bronchus Zhou, X. E. et al. a Photo-activation of Bpa by UV light. Luciferase activities were measured using a Omega luminometer (BMG LABTECH, Ortenberg, DE) equipped with two injectors. The five models featuring minimal energy of the imposed restraints for each of the templates showed very similar conformations and energies (Supplementary Fig. Identify the bone that articulates distally with the highlighted structure in the above ima. Are the highlighted vessels carrying oxygenated or deoxygenated blood? What structure receives blood from the venae cavae? A similar arrangement with analogous interactions was observed in the 2V2R-Gs-arr2 megaplex (PDBID: 6ni2)59 (see Supplementary Fig. Cys was systematically incorporated throughout the ICL2, the ICL3 (including the intracellular tips of TM5 and TM6), and the whole stretch starting from the intracellular tip of TM7, across helix VIII and the two phosphorylation clusters in the C-tail, up to P553, for a total of 120 positions. PubMed Central A: BSE: Backscattered electron imaging mode, image . The stadium had become home to more than 30,000 bats. PubMed The highlighted epithelium, when lining a serous membrane, is called a(n) _____. J. Biol. (2), (3), (4), respectively. Biol. In general, the orientations are limited by the membrane anchoring of C-edge with pitch angle showing a modest ~13 degrees deviation, followed by ~17 degrees of roll and up to 25 degrees of yaw deviations. \text { antispasmodics } & \text { ESWL } & \text { lower Gl series } & \text { stool guaiac } \\ Biol. Students also viewed. The phosphorylation sites in the distal cluster have been termed key sites, as they are essential for the formation of a high-affinity arrestin-receptor complex58. Nature Communications (Nat Commun) Sep. 2016. Identify the highlighted structures. Images. For illustrative purposes, a sphere is placed on C of labeled arr2 residues instead of the C where the distances are measured. For PCR, Phusion High Fidelity Polymerase was used (New England Biolabs GmbH). A) zona fasciculata Cell Res 29, 971983 (2019). However, structural studies of GPCR-arrestin complexes are hampered by their highly dynamic nature. D) branched, multinucleate cells with striations. 267, 85588564 (1992). C) leaving the heart to go to the systemic circuit A) renal pelvis Thank you for visiting nature.com. We'll bring you back here when you are done. 473, 41734192 (2016). Biol. Total views 100+ The pitch, roll, yaw angles are the angles to align the principal axes of the arrestin \({{{{{{\bf{X}}}}}}}_{{Arr}}\) to that of the receptor \({{{{{{\bf{X}}}}}}}_{{PTH}1R}\). Role of beta-arrestin in mediating agonist-promoted G protein-coupled receptor internalization. B) large intestine For SDS-PAGE, 4L of supernatant was incubated for 30min at 37C in LDS-Sample buffer (250mM Tris-HCl pH 8.5, 2% (w/v) LDS, 150mM DTT, 0.4mM EDTA, 10% (v/v) glycerol and 0.2mM Coomassie Brilliant Blue G). Distinct conformations of GPCRbeta-arrestin complexes mediate desensitization, signaling, and endocytosis. Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser. 41 as described below. Both plots are colored by the crosslinking yield of individual pairs. We further reveal the existence of a previously unappreciated interaction network that stabilizes phosphorylation sites downstream of the key sites. What is this muscle called? GPCR-mediated -arrestin activation deconvoluted with single-molecule precision. A beam of electrons moving in the positive x x -direction encounters a potential barrier that is 2.51 \mathrm {eV} 2.51eV high and 1.00 \mathrm {~nm} 1.00 nm wide. Identify the highlighted cells. conceived and supervised the computational part of the project. Among these, 443 pairs gave detectable crosslinking signals in western blots (Supplementary Figs. Reported to North America / Corporate Audit Director. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Mol. Pick 3 of the following and argue that the following are e Kim 2 A) kidney 110, 465502 (2006). If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Identify the highlighted structures. In the central crest, major hits were found in the finger loop, in the adjacent -strand VI (Fig. _____ _____ spinal cord anterior (ventral) root 2) Identify the highlighted structure. A) medulla Q: Identify the highlighted structures. The last PTH1R residue involved in a crosslinking pair was P524. BMC Evol. One day before transfection, 2.7106 HEK293T cells were seeded in a 10cm dish using full DMEM. A typical plant body mainly. For rhodopsin-arr1, ahomology model of arr2 was constructed fromthe arr1 structure usingthe sequence from UniProt ID: Q03431-1 [https://www.uniprot.org/uniprotkb/Q03431/entry#sequences]. and Y.A. The highlighted structure is a remnant of which structure in the fetus? designed and supervised the large-scale synthesis of BrEtY. C) renal medulla Nature 497, 137141 (2013). indicate whether each is a direct object, an indirect object, an objective complement, a predicate nominative, or & predicate adjective. Identify the highlighted structure. PubMed Biol. Figure5df shows a modest fluctuation of 1325 degrees in the orientation during MD simulations (Supplementary Data7), with the pitch variations being themost limited by the membrane anchoring of arr2 C-edge (Supplementary Fig. Arrestin interactions with G protein-coupled receptors: Direct binding studies of wild type and mutant arrestins with rhodopsin, 2-adrenergic, and m2 muscarinic cholinergic receptors. Identify one of these. These BrEtY-arr2 mutants were co-expressed with wt PTH1R, which carries four native Cys residues in the juxtamembrane intracellular elements (Supplementary Fig. Crystal structure of cone arrestin at 2.3: Evolution of receptor specificity. $$ This supports close similarities between rhodopsin- and secretin-like GPCR interactions with arrestins, while suggesting that the NTS1R-arr2 structures, where arrestin is positioned in a nearly perpendicular orientation, either represent a distinct type of arrestin engagement or reflect specific experimental conditions, as discussed in ref. D) filters incoming air, Identify the structure labeled "b." Identify the epithelium indicated by the arrows. Transfection was performed using PEI as described above. D) AB+, Blood in the pulmonary veins is: Under my leadership, I was responsible for advising customers on various personal banking and financial products and services in order to identify suitable solutions that met their financial goals. Identify the highlighted cells. The authors declare no competing interests. Solution for Identify the highlighted structure. Nguyen, A. H. et al. Notably, the arrestin orientation relative to GPCRs in these structures varies, suggesting that our understanding of arrestin binding to GPCRs is far from comprehensive. A) ovary It contains either a phosphorylation cluster, as in the PTH1R (proximal cluster), or negatively charged residues in many GPCRs58. Lead QIPP Analyst for Berkshire East CCGs. The first class of connective tissue system is fibroblast, which is a cell that constructs the structural fibers and ground substance of connective tissue. 23. Images. These mutations did not substantially affect receptor function or arrestin recruitment (Supplementary Fig. Lektion 1. Nat. Which type of secretion involves the loss of apical cytoplasm? 10). Experts are tested by Chegg as specialists in their subject area. et al. The intermolecular -sheet is stabilized both by backbone interactions and by a network of ionic interactions between the phosphate groups in PTH1R and basic residues of arr2 (Fig. SoftwareX 12, 1925 (2015). designed and performed all crosslinking experiments, and interpreted results. 5df, Supplementary Data7). EC50 values were calculated as mean with the appropriate CI from at least three independent experiments, each performed in quadruplicate. Highlighted region is elastic cartilage. A) small intestine Which structure is highlighted? The meetings highlighted the importance of institutional structures, capacity, policy implementation and data collection and analysis. Pairwise C-C distances were measured; when glycine was present, C was used instead. 21. Is the highlighted structure found on both the right and left lung? The entire model then underwent alternating rounds of energy-based optimization (Metropolis Monte Carlo and gradient descent) with and without harmonic restraints using Biased Probability Monte Carlo approach63 until convergence for a maximum of two million steps. (b) $V_2=0$. After a thorough screening of the arr2 surface via a photo-crosslinker, which revealed the footprint of the receptor on the arrestin, proximity-enabled crosslinking at 621 PTH1R-arr2 intermolecular pairs of amino acids provided 136 statistically validated proximity points throughout the large interaction interface. Identify the highlighted organ. 34, 04103, Leipzig, Germany, Yasmin Aydin,Thore Bttke,Stefan Ernicke,Anna Fortmann&Irene Coin, Department of Quantitative and Computational Biology, University of Southern California, Los Angeles, CA, USA, Medical Faculty, Institute for Drug Discovery, Leipzig University, Bruederstr. Bands appearing below 55kDa are due to intramolecular crosslinking, while other bands at higher MW likely belong to arrestin dimers (band at D78) or possibly other complexes39. 21 terms. PubMed Signal. 3a and Supplementary Data3), only 72 out of 139 pairs (~52%) fall within the strict 10.2 cutoff, which is the C-C distance in the BrEtY-Cys adduct (Fig. C) renal column B) medulla First, when crosslinking arr2 with full-length V2R, we did find strong crosslinking hits in the 160-loop, but not in the finger loop (Supplementary Fig. Please select the correct language below. Biochemistry 33, 56615673 (1994). LANSING, Mich. - Recently, Governor Whitmer signed a bipartisan supplemental to build more affordable housing, revitalize communities, and eliminate blight. The stacked histograms on top give an overall summarizing statistics of the C-C distances in each of the PTH1R regions showing that the vast majority of pairs stays within 15 distance during MD simulations. Struct. Mol. 69, 256297 (2017). Identify the highlighted structure. S.E. 33. 368, 375387 (2007). Cell 185, 16611675.e16 (2022). 40, D370D376 (2012). 2). This suggests that the 160-loop of arr2 is dynamically sandwiched between ICL3 and the C-tail of the receptor, which probably contributes to the stabilization of the overall organization of the complex. Nat. A: Bone is defined as the type of living tissue that play a major role in making up the skeleton system. 34, 04103, Leipzig, Germany, Division of Medicinal Chemistry, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Faculty of Science, Vrije Universiteit Amsterdam, De Boelelaan 1108, 1081 HZ, Amsterdam, The Netherlands, Department of Phar-macology, Vanderbilt University, Nashville, TN, 37232-0146, USA, Department of Chemistry, Bridge Institute, USC Michelson Center for Convergent Biosciences, University of Southern California, Los Angeles, CA, USA, You can also search for this author in Orlando, Florida, United States. Identify the highlighted cell. A.F. Our data clearly show that the ICL3 of PTH1R extensively interacts with arr2, tracing its path from the C-loop contacts (R312, E313, L315) through -strand VI (D78, V81) to the concave surface of the N-domain (K147) up to the 160-loop (K157). D) white blood cell, Which structure is highlighted? Proc. This represents the first computational model of Cyc2 that accounts for the membrane microenvironment and the acidity in the extracellular matrix. ChemBioChem 20, 683692 (2019). Identify the highlighted cells. This work was supported by the German Research Foundation (DFG Grants: CO822/3-1 and CO822/3-2 to IC; CRC 1423, project number 421152132, subproject B04 to IC), by the National Institute of Diabetes and Digestive and Kidney Diseases grant P01 DK011794 (VK), and the National Institute of General Medical Sciences (NIGMS) R35 GM122491 (VVG). Cells were co-transfected with three plasmids: (1) 900ng of a plasmid encoding arr2-stop codon mutant, (2) 900ng of XYPylRS/4xM15-tRNA (available from ADDGENE #155343)39, and (3) 300ng of a vector encoding indicated PTH1R construct. 1b, c), extended up to the distal tip of the convex side of arr2 as well as to -helix I (Fig. stratified squamous epithelium, keratinized. The highlighted structures are composed of what type of cartilage? A333V336), the vertical points to the intracellular side of membrane normal to the lipid bilayer. To analyze the dynamic accessibility of the residue pairs for crosslinking, as well as the overall conformational stability of the complex, we performed unbiased Molecular Dynamics (MD) simulations without crosslinking restraints. Sci. 31). Identify the highlighted structure. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. The results highlighted the variety of organisational structures, which tended to structure with the use of the device. Our approach does not require any modifications stabilizing the protein complex and reflects the dynamics of the natural system, allowing us to fill in the gaps in the flexible elements that are missing in known GPCR-arrestin structures. A) an indentation where urine collects before transport to the bladder EMBO Rep. 21, e50437 (2020). Source data are provided with this paper. performed the densitometric analysis of the data. amontoya052019. Identify the highlighted structure in green in the above image: 2. C) uvula D) protects the kidney by being a barrier, What urinary structure produces the urine filtrate? ICL3 and the C-terminus beyond L481 up to G530) and arr2 were remodeled to their native sequences. -Su Use LEFT and RIGHT arrow keys to navigate between flashcards; Use UP and DOWN arrow keys to flip the card; What term is used to refer to the highlighted epithelium in a blood vessel? Biomolecules 11, 218 (2021). The highlighted structure is made of what type of cartilage? 17, 163 (2017). 406, 467478 (2011). 4b, Supplementary Fig. 5df are provided as Supplementary Data7. The highlighted structure is contained within which bone? Although in the structure of arr2 bound to the V2Rpp the proximal phosphorylation lies close to the finger loop, the resolved segment of the receptor C-tail in the full-length V2R-arr2 structure25 guides the proximal cluster to the arrestin N-edge, in line with our crosslinking results with the full-length V2R (Supplementary Fig. Identify the highlighted structure. Boc-l-Tyr-OMe and 26mL (0.30mol, 5.0 eq.) 13C-NMR: [ppm, d4-MeOH, 22C]=171.5, 162.7, 159.4, 131.7, 128.2, 120.1, 69.4, 55.5, 36.6, 30.5. Transcribed image text: Identify the highlighted structures. Identify the anatomical feature of the frontal section of the heart labeled D. pectinate muscles cusp of the bicuspid valve trabeculae carneae cusp of the tricuspid valve Identity the pituitary normone iabeied I ADH LSH LH OXT Identify the highlighted sheep heart structure (frontal section). Another unresolved region in all existing structures of GPCR-arrestin complexes is located upstream of the key phosphorylation sites. up to 910 from the C)37. bd Surface representation of receptor-bound bovine arr2 (PDBID: 4jqi)70 with crosslinking hits highlighted in red; the insets are representative western blots of whole cell lysates from photo-crosslinking experiments (n=1) detected with an -HA antibody (comprehensive overview in Supplementary Fig. J. Biol. C) antibodies phagocutize the foreign antigen 1957, 38 (2019). B) right inferior lobe the 21th century! 2b) were translated into 136 soft harmonic restraints weighted by the corresponding crosslinking yields in an integrative modeling framework47,48,49. 2a, 3). a) parafollicular cell HIGHLIGHTS - Implemented a new organizational structure for Operations & Logistics; completed 6 months early - Established DOT/FRA Compliance Framework to resolve 30+ regulatory non-conformances . The highlighted epithelium, when found lining a blood vessel, is called a(n). A) right atrium Identify the highlighted structures. CAS 5 Identify the highlighted structure 6 Identify the highlighted structure from SC 131 at Kaplan University, Davenport. C) internal iliac artery Biosyst. & Abou-Samra, A. B) stomach What is a text file and what is a binary file? Integr. An initial PTHLA-PTH1R model was extracted from a cryo-EM model of thePTHLA-PTH1R-Gs complex (PDBID: 6NBF)33; missing loops and side-chains were added using loop modeling and optimization tools in ICM-Pro v.3.9.2c. Nature 583, 862866 (2020). Gurevich, V. V. et al. A) A+ A) laryngopharynx Penis. Plasma. Google Scholar. Arrestins are cytosolic proteins that regulate signaling of nearly all G protein-coupled receptors (GPCRs)1,2. D) labia minora, Which zone of the adrenal cortex is most superficial? D) subclavian. Sutton, R. B. et al. J. Biol. 4d). Primary antibodies were diluted in 5% NFDM in TBS-T as follows: rat -HA 3F10 (Roche Diagnostics, Mannheim, Germany) 1:2000; mouse -PTH1R 4D2 (Thermo Fisher Scientific) 1:2000. After 24h, cells were stimulated at 37C for 3h by the addition of 25l of PTH(1-34) dissolved in pure DMEM to final concentrations from 1012 to 106M in a 96-well. 29, 18591865 (2008). Coin, I. et al. Faculty of Life Sciences, Institute of Biochemistry, Leipzig University, Bruederstr. -Compact tissue is the outer layer of bones and is harder than the other layers. Afterward, 50l of 16.8M coelenterazine h dissolved in BRET buffer were added to the wells and incubated for 10min at 37C. MD trajectories were analyzed using MdTraj package68. D) palatine tonsil, Which digestive structure absorbs water and consolidates waste? Biol. A) leukocyte & Vilardaga, J.-P. International Union of Basic and Clinical Pharmacology. Fibroblast. Introduction to Robotics: Mechanics and Control (Pearson Education, Inc., 2004). (e) $\overrightarrow{\mathbf{v}}_1$ and $\overrightarrow{\mathbf{v}}_2$ are in the same plane. Biol. Identify the highlighted lobe of the lung. B) membranous urethra errors: I remeber watching the D) renal column, Which structure is the location where sperm maturation occurs? C) seminal vesicle mickey Mouse club show I mean Proc. 5). 9), which does not interact with the receptor core43. Byron Almen, Dorothy Payne, Stefan Kostka, Kulik & Roberson article - Week 7 - Diversity. Distances in the starting model are marked with crosses. Article Chem. Question: PAL: Models > Nervous System - Special Senses > Lab Practical > Question 24 Part A Identify the highlighted structure. A., Barak, L. S. & Caron, M. G. Association of beta-arrestin with G protein-coupled receptors during clathrin-mediated endocytosis dictates the profile of receptor resensitization. 18a), is reconcilable with our crosslinking data. Led team of 5 professionals. Curr. 18c) and with our PTH1R-arr2 model. 18b). All MD simulations were conducted with Gromacs (v.2020.1) simulation engine66 under Charmm36 force field parameters and topologies67. D) cortex. 3a, Supplementary Data3, and Supplementary Figs. Get the most important science stories of the day, free in your inbox. . Cells were passaged at ~80% confluence. Google Scholar. 114, 25622567 (2017). BrEtY was synthesized according to ref. Hall, M. P. et al. Central Canal. all of we kids in the tV audience Together these two systems are primarily responsible for maintaining. The lower bound of thecovalent diameter at 10.2 of BrEtY is drawn as a thick vertical dash line accompanied by a thin dash line at 15. 16. A) axillary and lateral thoracic First week only $4.99! -Liaising Onsite with Business as well as IT executives. Indeed, while only about half of the pairwise crosslinks strictly satisfy the 10.2 Cys-BrEtY C-C distance in the static model, most of them (96%) come within this distance at some point during unbiased MD simulations, with the six outliers coming within 15. A) moistens and warms incoming air Tawfeek, H. A. W., Qian, F. & Abou-Samra, A. The luminesence of Renilla luciferase was detected using a 47530nm emission filter. This work assessed the use of WorldView-3 data, Very High-Resolution Airborne Images, and Structure from Motion point clouds to identify the status of citrus parcels using two machine learning algorithms: Random Forest and Support Vector . Back. Source data for Fig. The receptor C-terminus downstream of the distal cluster (res. 2). Which component of the connective tissue in this field of view is highlighted? ESI-MS: C11H15Br1N1O3+, Mcalc: 288.0230, Mfound=288.0239. This model predicts several details of PTH1R-arr2 interactions. 10, 1261 (2019). ADS Merkley, E. D. et al. Francisco has a combination of commercial leadership, team building, coaching, dedication, and mentoring skills that elevate his teams to achieve high performance. The results show that mean annual precipitation and soil pH are the major environmental factors that shape soil bacterial communities in maize soils and members of the core microbiome were more frequently found in the center of the ecosystem network compared with other taxa. 72 members of staff were interviewed to address repetitive patterns and clusters within their organisations . Samples were resolved on 8% Tris/glycine polyacrylamide gels and transferred to a PVDF membrane (Millipore Immobilon, Merck, pore size 0.45m). Identify the highlighted structure of the trachea. 6 and 7). Cells were lysed in 80L Triton lysis buffer (50mM HEPES pH 7.5, 150mM NaCl, 10% glycerol, 1% Triton X-100, 1.5mM MgCl2, 1mM EGTA, 1mM DTT and 1 protease inhibitor) for 30min on ice and vortexed every 10min. Inferior. And with hiring managers looking at each resume for an average of six seconds, you'd better believe you need to go the extra mile to convince them to give you the time of day. The complex of DNA and protein within the highlighted structure is known as _____. Cardiovascular A&P. 104 . Distinct G protein-coupled receptor phosphorylation motifs modulate arrestin affinity and activation and global conformation. The coordinates of the best PTH1R-arr2 model, based on the M2R-arr2 template, generated in this study have been deposited in the ModelArchive database under accession code ma-2b2xn, other models are deposited under ma-33nf3, ma-5ui3z, ma-9mi1q, ma-f1hkg, ma-v0m33.

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